ythdf3 antibody (ip) Search Results


ythdf3 antibody (ip)  (ABclonal Biotechnology)
90
ABclonal Biotechnology ythdf3 antibody (ip)
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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ythdf3 santa cruz sc377119 ip  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ythdf3 santa cruz sc377119 ip
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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proteintech 24744 1 ap ythdf3 wb  (Proteintech)
96
Proteintech proteintech 24744 1 ap ythdf3 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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pfn1 santa cruz sc137235 ip  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology pfn1 santa cruz sc137235 ip
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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ip 4ug sample proteintech 18277 1 ap 286  (Proteintech)
94
Proteintech ip 4ug sample proteintech 18277 1 ap 286
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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alkbh5 wb  (Proteintech)
96
Proteintech alkbh5 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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ythdf1 proteintech  (Proteintech)
96
Proteintech ythdf1 proteintech
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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smmhc proteintech 21404 wb  (Proteintech)
94
Proteintech smmhc proteintech 21404 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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abcam ab128175 igf2bp3 wb  (Proteintech)
96
Proteintech abcam ab128175 igf2bp3 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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proteintech 22803 1 ap igf2bp2 wb  (Proteintech)
96
Proteintech proteintech 22803 1 ap igf2bp2 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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proteintech 14642 1 ap hif 1α wb  (Proteintech)
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Proteintech proteintech 14642 1 ap hif 1α wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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abcam ab220161 igf2bp1 wb  (Proteintech)
97
Proteintech abcam ab220161 igf2bp1 wb
FTO regulates FOXO6 m6A modification via <t>YTHDF3-dependent</t> manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .
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Image Search Results


FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .

Journal: Frontiers in Pharmacology

Article Title: Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms

doi: 10.3389/fphar.2023.1260300

Figure Lengend Snippet: FTO regulates FOXO6 m6A modification via YTHDF3-dependent manner. (A) Effect of FTO overexpression and knockdown on m6A level of FOXO6 detected by MeRIP-qPCR; (B) The potential m6A sites in FOXO6 predicted by SRAMP website; (C) The secondary RNA structure and location of m6A site on FOXO6 mRNA; (D) The m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (E) Effect of FTO overexpression on m6A level of FOXO6 detected by MeRIP-qPCR after methylation site mutation; (F) The expression level of m6A recognition protein YTHDF3 detected by Western blot (1:Control; 2:Model); (G) RIP‐qPCR detection of the binding relationship between YTHDF3 and FOXO6; (H) The effect of YTHDF3 knockdown on FOXO6 expression detected by RT-qPCR; (I) The effect of YTHDF3 knockdown on FOXO6 mRNA stability detected by actinomycin D assay. * p < 0.05 ; ** p < 0.01 .

Article Snippet: HGMC lysate samples were incubated with either YTHDF3 antibody (IP) (0202730101, ABclonal, Wuhan, China) or control IgG antibody (IgG) at 4°C for 16 h. Finally, total RNA was extracted and analysed using RT-qPCR assay.

Techniques: Modification, Over Expression, Knockdown, Methylation, Mutagenesis, Expressing, Western Blot, Control, Binding Assay, Quantitative RT-PCR

The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.

Journal: Frontiers in Pharmacology

Article Title: Overexpression of FTO inhibits excessive proliferation and promotes the apoptosis of human glomerular mesangial cells by alleviating FOXO6 m6A modification via YTHDF3-dependent mechanisms

doi: 10.3389/fphar.2023.1260300

Figure Lengend Snippet: The binding relationship between YTHDF3 and FOXO6 predicted by RM2Target website.

Article Snippet: HGMC lysate samples were incubated with either YTHDF3 antibody (IP) (0202730101, ABclonal, Wuhan, China) or control IgG antibody (IgG) at 4°C for 16 h. Finally, total RNA was extracted and analysed using RT-qPCR assay.

Techniques: Binding Assay, Modification